- Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells
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Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells
United Chemical Technologies, or UCT, led the way in developing new SPE products in 2021. The company introduced a new polymeric SPE in the 96-well plate format with Micro-Prep. The Micro-Prep utilized disk technology with dense packing of the sorbent particles to generate the uniform flow needed for sample clean-up and analyte enrichment.
An improved boosting to amplify signal with isobaric labeling strategy for precise quantitative single-cell proteomics. Samples in both nanowell chip and N2 chip were completely dried out in a vacuum desiccator and stored in a −20 °C freezer until analysis. Three leukemia cell lines (MOLM-14, K562, and CMK) were kindly provided by Dr. Anupriya Agarwal at Oregon Health & Science University. A A 3D illustration and a photo of the N2 chip.
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Finally, TMT labeled boost and reference (0.5 ng) peptides were distributed into nanowells. The samples were acidified with 5 nL of 5% FA and dried for long-term storage. We analyzed a total of 108 single cells from three murine cell lines, including epithelial cells , immune cells (Raw264.7), and endothelial cells (Fig.3a). Noteworthily, these three cell https://turbo-tax.org/ types have different sizes, which allows us to evaluate if the workflow presents a bias in protein identification or quantification based on cell sizes. Specifically, Raw cells have a diameter of 8 µm, SVEC of 15 µm and C10 of 20 µm (Fig.S3a). Herein, we describe a nested nanoPOTS chip to improve the isobaric-labeling-based scProteomics workflow.